CD8a Antibody,MA90692

Description

CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8a is a 34 kD protein that belongs to the immunoglobulin family. The CD8 α/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR α/β T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 α/α homodimer is expressed on a subset of γ/δ TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.

The 53-6.7 antibody is a well-characterized monoclonal antibody that targets CD8a, aglycoprotein found on the surface of cytotoxic T cells and some dendritic cells. Below is asummary of its key features and applications based on the information provided:

Key

Features:

1. Competes with clone 5H10-1: The 53-6.7 antibody competes with the 5H10-1 antibody forbinding to thymocytes, suggesting overlapping or closely related epitopes on CD8a.

2.Functional Effects:

-Blocks antigen presentation via MHC class 1.

-Inhibits T cell responses to IL-2.

-Used for depletion of cD8a+ cells in vivo and in vitro.

3. Applications:

-Immunoprecipitation: Used for isolating CD8a proteins or complexes.

-Cell Depletion: Effective for in vivo and in vitro depletion of CD8a+ cells.

-Inhibition ofT Cell Proliferation: Blocks CD8 T cell proliferation.

-Blocking Cytotoxicity: Inhibits cytotoxic activity of CD8+ T cells.

-Immunohistochemistry (HC):

Works on acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections.

Not recommended for formalin-fixed paraffin sections.Spatial Biology: Used in techniques like IBEX (lterative Bleaching Extends multiplexity) for0high-dimensional tissue imaging.

Reactivity
Rat IgG2a, κ Anti Mouse

Applications in Detail

1. Immunoprecipitation: Used to isolate CD8a or its associated proteins for downstreamanalysis.

2. Cell Depletion: Effective for removing CD8a+ cells in experimental models to study their rolein immune responses.

3. Inhibition of T Cell Function: Blocks CD8 T cell proliferation and cytotoxicity, making it usefulfor studying T cell-mediated immune responses.

4. Immunohistochemistry: Suitable for staining acetone-fixed frozen sections and zinc-fixedparaffin-embedded sections, but not formalin-fixed paraffin sections.

5. Spatial Biology: Used in advanced imaging techniques like IBEX to study the spatialdistribution of CD8a+ cells in tissues.